PARP12 Antibody

• Catalog Number: orb19172
• Category: Antibodies
• Description: Goat polyclonal to PARP12
• Target: PARP12
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NAHDIKNKSSAP
• RRID: AB_10747416
• MW: 79.1
• Tested applications: ELISA, IF
• Dilution range: ELISA: 1:128000, WB: 0.03 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 110kDa band in Human Liver lysates and in lysates of hepatoblastoma cell line HEPG2 after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.1kDa according to NP_073587.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PARP12 antibody, anti poly (ADP-ribose) polym
• Note: For research use only
• Entrez: 64761