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PARP12 Antibody

SKU: orb19172

Description

Goat polyclonal to PARP12

Images & Validation

Tested ApplicationsELISA, IF
Dilution RangeELISA: 1:128000, WB: 0.03 μg/ml
ReactivityHuman
Predicted ReactivityCanine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 110kDa band in Human Liver lysates and in lysates of hepatoblastoma cell line HEPG2 after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.1kDa according to NP_073587.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetPARP12
Protein SequenceNAHDIKNKSSAP
Molecular Weight79.1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti PARP12 antibody, anti poly (ADP-ribose) polymerase family, member 12 antibody, anti HGNC:21919 antibody, anti FLJ22693 antibody, anti PARP-12 antibody, anti ZC3H1 antibody, anti ZC3HDC1 antibody, anti zinc finger CCCH type domain containing 1 antibody, anti zinc finger CCCH-type domain containing 1 antibody

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PARP12 Antibody (orb19172)

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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