Pan Cytokeratin (Acidic and Basic) Antibody

SKU: orb1252156

Description

Pan Cytokeratin (Acidic and Basic) Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1ug/10e6 cells in 0.1mlIF: 1-2 µg/mLWB: 0.5-1 µg/mLIHC (FFPE): 0.25-0.5 µg/mL for 30 min at RTPrediluted format: incubate for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant full-length human KRT76 and KRT77 proteins were used as the immunogen for this pan Cytokeratin antibody.
• Target: KRT1, KRT3, KRT4, KRT5, KRT6A, KRT8, KRT10, KRT14, KRT15, KRT16, KRT19
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human skin with pan Cytokeratin antibody. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Quick Database Links

Gene Symbol

KRT1, KRT3, KRT4, KRT5, KRT6A, KRT8, KRT10, KRT14, KRT15, KRT16, KRT19

UniProt

P19013, P19012, P13647, P02533, P48668, P08727, P13645, P08779, P04264, P05787, P12035