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Catalog Number | orb1247929 |
---|---|
Category | Antibodies |
Description | PACSIN1 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Canine, Porcine |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: SSSYDEASLAPEET-C |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 51.1kDa according to NP_065855.1). Recommended concentration: 0.03-0.1ug/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex and Cerebellum). Recommended concentration: 2.5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | PACSIN1 |
UniProt ID | Q9BY11 |
NCBI | NP_065855.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | PACSIN1, protein kinase C and casein kinase substr Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 51.1kDa according to NP_065855.1). Recommended concentration: 0.03-0.1ug/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex and Cerebellum). Recommended concentration: 2.5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247929 (2.5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1247929 (1 ug/ml) staining of Hippocampus) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1247929 (2.5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC, WB | |
Bovine, Canine, Human, Porcine | |
Goat | |
Polyclonal | |
Unconjugated |
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