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Catalog Number | orb1928619 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB3586 |
Tested applications | IHC-P, WB |
Predicted Reactivity | Other |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 50966 Da |
Target | This PACSIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-38 amino acids from the N-terminal region of human PACSIN1. |
UniProt ID | Q9BY11 |
NCBI | NP_001186512.1, NP_065855.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Protein kinase C and casein kinase substrate in ne Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of anti-PACSIN1 Pab in HL-60 cell line lysate. PACSIN1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of anti-PACSIN1 Pab in mouse brain tissue lysate.PACSIN1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of PACSIN1 (arrow) using rabbit polyclonal PACSIN1 Antibody (G23). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PACSIN1 gene.
Western blot analysis of lysate from human heart tissue, using PACSIN1 Antibody (G23). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
Western blot analysis of lysates from human brain, mouse brain and rat brain tissue lysate (from left to right), using PACSIN1 Antibody (G23). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
Western blot analysis of lysates from Daudi cell line, human heart and pancreas tissue lysate (from left to right), using PACSIN1 Antibody (G23). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
Western blot analysis of lysates from Daudi cell line, human brain, human pancrease, human heart tissue (from left to right), using PACSIN1 Antibody (G23). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.