PACSIN1 Antibody

SKU: orb18842

Description

Goat polyclonal antibody to PACSIN1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.03-0.1 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 50kDa band observed in Human Brain (hippocampus) lysates (calculated MW of 51.1kDa according to NP_065855.1). Recommended concentration: 0.03-0.1 μg/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PACSIN1
• Molecular Weight: 51.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti PACSIN1 antibody, anti KIAA1379 antibody, anti SDPI antibody, anti syndapin I antibody

Western blot analysis of Hippocampus lysate using PACSIN1 antibody

Immunohistochemical staining of Human Cerebellum using PACSIN1 antibody

Immunohistochemical staining of Human Cortex using PACSIN1 antibody

Quick Database Links

Gene Symbol

PACSIN1

NCBI Gene ID

29993