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Osteopontin antibody
Description
Images & Validation
−| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | IHC-P: 1:50-500, WB: 1:200-1000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Osteopontin. Please contact us for the exact immunogen sequence. The peptide is available as orb13218. |
| Target | Osteopontin |
| Molecular Weight | 35 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC-P staining of rat kidney tissue using Osteopontin antibody (2.5 ug/ml)

Immunohistochemical staining of human ovarian cancer tissue using anti-Osteopontin (2.5 ug/ml)

Western blot analysis of rat kidney (lane 1), mouse brain (lane 2), mouse testis (lane 3), human breast tumor (lane 4), human ovarian cancer (lane 5); u251 cells (lane 6) tissue using Osteopontin antibody (1 ug/mL)

Immunohistochemical staining of human ovarian cancer tissue using Osteopontin antibody (2.5 ug/ml)
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Protocol Information
Osteopontin antibody (orb385622)
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