SPP1 Antibody (C-term)

SKU: orb1929992

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 3

Tested Applications	FC, IHC-P, WB
Dilution Range	WB - 1:2000, IHC-P - 1:50-100, FC - 1:10-50
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	BTD30179
Immunogen	This SPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-301 amino acids from the C-terminal region of human SPP1. Antigen Region: 273-301 aa.
Target	SPP1
Molecular Weight	35423 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, SPP1, BNSP, OPN

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Flow cytometric analysis of NCI-H292 cells using SPP1 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma with SPP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-SPP1 Antibody (C-term) at 1:2000 dilution. Lane 1: Human plasma lysate. Lane 2: Human placenta lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.