SPP1 Antibody (C-term)

• Catalog Number: orb1929992
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18957
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 35423 Da
• Target: This SPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-301 amino acids from the C-terminal region of human SPP1.
• UniProt ID: P10451
• NCBI: NP_001238759.1, NP_000573.1, NP_001238758.1, NP_001035147.1, NP_001035149.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Osteopontin, Bone sialoprotein 1, Nephropontin, Se
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of NCI-H292 cells using SPP1 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma with SPP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-SPP1 Antibody (C-term) at 1:2000 dilution. Lane 1: Human plasma lysate. Lane 2: Human placenta lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.