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Osteopontin Rabbit Polyclonal Antibody

Catalog Number: orb11192

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb11192
CategoryAntibodies
DescriptionOsteopontin Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityHuman, Rat
ReactivityHuman, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from mouse OPN (221-280/294aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:500-2000, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW34 kDa
TargetSPP1
UniProt IDP10923
RRIDAB_10920784
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesBNSP; Bone sialoprotein 1; BSP I; BSPI; Early T ly
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NoteFor research use only
Expiration Date12 months from date of receipt.
Osteopontin Rabbit Polyclonal Antibody

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-Osteopontin antibody (orb11192), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Osteopontin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Osteopontin) Polyclonal Antibody, Unconjugated (orb11192) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Osteopontin Rabbit Polyclonal Antibody

Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug, Lane 2: A549 (Human) Cell Lysate at 30 ug, Lane 3: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-Osteopontin (orb11192) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 60 kD.

Osteopontin Rabbit Polyclonal Antibody

Tissue/Cell: rat shin bone, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-osteopontin Polyclonal Antibody, Unconjugated (orb11192) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • Osteopontin Rabbit Polyclonal Antibody [orb11191]

    FC,  IF,  IHC-Fr,  IHC-P,  WB

    Rat

    Human, Rat

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 50 μl
  • Osteopontin Rabbit Polyclonal Antibody [orb500935]

    FC,  IF,  IHC-Fr,  IHC-P,  WB

    Human

    Human, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
  • OPN-a/b Antibody (N-term) [orb1938103]

    IF,  IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • SPP1 Rabbit Polyclonal Antibody [orb576067]

    IHC,  WB

    Bovine, Canine, Goat, Guinea pig, Mouse, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • OPN rabbit pAb [orb765958]

    ELISA,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl