OCLN Antibody

• Catalog Number: orb1239676
• Category: Antibodies
• Description: OCLN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: OCLN antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN.The immunogen is located within the last 50 amino acids of OCLN.
• Concentration: 1 mg/mL
• Dilution range: OCLN antibody can be used for detection of OCLN by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 57 kDa Observed: 57 kDa
• Target: OCLN
• UniProt ID: Q16625
• NCBI: AAH29886
• Storage: OCLN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: OCLN Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: OCLN Antibody: BLCPMG, Occludin
• Note: For research use only
• Application notes: OCLN antibody can be used for detection of OCLN by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of OCLN in human liver tissue lysate with OCLN antibody at 1 µg/mL.

Immunohistochemistry of OCLN in human liver tissue with OCLN antibody at 2.5 µg/mL.

Immunofluorescence of OCLN in Human Liver tissue with OCLN antibody at 20 µg/mL.

Immunofluorescence of OCLN in human liver tissue with OCLN antibody at 20 µg/ml. Red: OCLN Antibody (orb1239676) Blue: DAPI staining.

Immunofluorescence of OCLN in mouse brain tissue with OCLN antibody at 20 µg/mL. Red: OCLN Antibody (orb1239676) Blue: DAPI staining.

Immunohistochemistry of OCLN in mouse brain tissue with OCLN Antibody at 5 µg/mL.