Occludin Rabbit Polyclonal Antibody

SKU: orb1294255

Description

Rabbit polyclonal antibody to Occludin

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:500-1:1500, Immunofluorescence 1:200–1:300, Immunohistochemistry 1:100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human Occludin / OCLN
• Target: Occludin
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

anti-Occludin antibody - at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294255 overnight at 4°C.

anti-Occludin antibody - at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294255 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling Occlidin with orb1294255 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling Occlidin with orb1294255 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Quick Database Links

Gene Symbol

Occludin