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Catalog Number | orb1538187 |
---|---|
Category | Antibodies |
Description | NT5E/eNT/CD73 Antibody |
Target | NT5E / eNT / CD73 |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | IgG1 |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | Ascitic fluid, 0.03% sodium azide. Ascitic fluid, 0.03% sodium azide |
Immunogen | Purified recombinant fragment of NT5E expressed in E. coli. |
Tested applications | ELISA, IHC, IHC-P |
Dilution range | ELISA (1:10000), IHC, IHC-P (1:200 - 1:400) |
Application notes | Further information: Immunohistochemistry: LS-B3897 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3897 was determined to be 1:200. |
Clone Number | 1D7 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | NT5E, 5-nucleotidase, ecto (CD73), 5'-nucleotidase Read more... |
Note | For research use only |
ELISA, IF, IHC, IHC-P, SDS-PAGE, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |