NT5E/eNT/CD73 Antibody

• Catalog Number: orb1536272
• Category: Antibodies
• Description: NT5E/eNT/CD73 Antibody
• Target: NT5E / eNT / CD73
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Buffer/Preservatives: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol. PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 290-550 of human NT5E (NP_002517.1). LKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKFST
• Tested applications: IF, IHC, IHC-P, WB
• Dilution range: IF (1:50 - 1:200), IHC, IHC-P (1:100), WB (1:500 - 1:2000)
• Application notes: Further information: The predicted MW is 57kDa/63kDa, while the observed MW by Western blot was 69kDa.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NT5E, 5-nucleotidase, ecto (CD73), 5'-nucleotidase
• Note: For research use only

Immunofluorescence analysis of MCF7 cell using NT5E antibody. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines.