NT5E/eNT/CD73 Antibody

• Catalog Number: orb1537931
• Category: Antibodies
• Description: NT5E/eNT/CD73 Antibody
• Target: NT5E / eNT / CD73
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b,k
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS, pH 7.4, 0.1% Sodium Azide. PBS, pH 7.4, 0.1% Sodium Azide
• Immunogen: Recombinant human CD73 (27-252aa) purified from E. coli.
• Tested applications: ELISA, IF, IHC, IHC-P, SDS-PAGE, WB
• Dilution range: ELISA, IF, IHC, IHC-P (10 µg/ml), SDS-PAGE, WB (1:1000 - 1:2000)
• Application notes: Further information: Immunohistochemistry: LS-B2943 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2943 was determined to be 10 ug/ml.
• Clone Number: 2B6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NT5E, 5-nucleotidase, ecto (CD73), 5'-nucleotidase
• Note: For research use only

IF (ICC) notes: ICC/IF analysis of CD73 in HeLa cells. The cell was stained with CD73 antibody (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Cell lysates of human mesenchymal stem cell (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD73 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL.

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD73 antibody (1:1000).