NOX1 Antibody

• Catalog Number: orb1249345
• Category: Antibodies
• Description: NOX1 Antibody
• Target: NOX1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: The immunogen for this antibody is: C-DKATDIVTGLKQK
• UniProt ID: Q9Y5S8
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in lysates of cell line epithelial colorectal adenocarcinoma and HeLa (calculated MW of 59kDa according to NP_39249.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 7.5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NOX1, NADPH oxidase 1, GP91-2, MOX1, NOH-1, NOH1,
• Note: For research use only
• NCBI: NP_39249.1, NP_008983.2
• Expiration Date: 12 months from date of receipt.

orb1249345 (7.5 ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1249345 (0.1 ug/ml) staining of lysate of cell line colorectal adenocarcinoma (A) and HeLa (B) (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249345 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.