Nox1 Rabbit Polyclonal Antibody

SKU: orb6469

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Description

Research Area

Cytotoxic Cells, DNA Damage / RNA Repair, Epigenetics and Nuclear Signaling, Immue System, Metabolism, Nucleotide Metabolism, Oxidative Stress

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Rabbit, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Nox1 (425-515/515aa)
Target	NOX1
Molecular Weight	62 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Mitogenic oxidase 1; MOX1; NADH/NADPH mitogenic oxidase subunit P65 MOX; NADPH oxidase 1; NADPH oxidase 1 variant NOH 1L; NADPH oxidase homolog 1; NOH 1; NOH1; RP1-146H21.1; GP91-2; Mitogenic oxidase(pyridine nucleotide dependent superoxide generating).

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Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Nox1 (orb6469) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-Nox1 (orb6469) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 62 kD, Observed band size: 57 kD.

Sample: Testis (Mouse) Lysate at 40 ug, Uterus (Mouse) Lysate at 40 ug, DU145 (Human) Cell Lysate at 30 ug, Primary: Anti-Nox1 (orb6469) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 60 kD.

Quick Database Links

Gene Symbol

NOX1

UniProt

Q9Y5S8

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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