NOTCH3 Antibody

SKU: orb1260379

Description

NOTCH3 Antibody

Research Area

Cell Biology, Epigenetics & Chromatin, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2200 to the C-terminus of human NOTCH3 (NP_000426.2).
• Target: NOTCH3
• Molecular Weight: Observed: 280kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NOTCH3, Notch homolog 3 (Drosophila), HGNC:7883, CADASIL, CASIL, Notch (Drosophila) homolog 3, Notch homolog 3, IMF2

Western blot analysis of extracts of various cell lines, using NOTCH3 antibody (orb1260379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Quick Database Links

Gene Symbol

NOTCH3

UniProt

Q9UM47