NOTCH3 Antibody

• Catalog Number: orb19112
• Category: Antibodies
• Description: Goat polyclonal antibody to NOTCH3
• Target: NOTCH3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QLGPQPEVTPKRQ
• RRID: AB_10747415
• MW: 244
• Tested applications: ELISA, FC, IHC
• Dilution range: ELISA: 1:16000, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.1. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti NOTCH3 antibody, anti Notch homolog 3 (Drosop
• Research Area: Cardiovascular Disease, Developmental Biology, Epi
• Note: For research use only
• Entrez: 4854

Immunohistochemical staining of Human Hippocampus using NOTCH3 antibody

Immunohistochemical staining of Human Placenta using NOTCH3 antibody

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using NOTCH3 antibody.