NOTCH3 Antibody

• Catalog Number: orb19112
• Category: Antibodies
• Description: Goat polyclonal antibody to NOTCH3
• Target: NOTCH3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QLGPQPEVTPKRQ
• RRID: AB_10747415
• MW: 244
• Tested applications: ELISA, FC, IHC
• Dilution range: ELISA: 1:16000, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.1. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti NOTCH3 antibody, anti Notch homolog 3 (Drosop
• Research Area: Cardiovascular Disease, Developmental Biology, Epi
• Note: For research use only
• Entrez: 4854
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Hippocampus using NOTCH3 antibody

Immunohistochemical staining of Human Placenta using NOTCH3 antibody

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using NOTCH3 antibody.