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Catalog Number | orb19112 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to NOTCH3 |
Target | NOTCH3 |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Protein Sequence | QLGPQPEVTPKRQ |
RRID | AB_10747415 |
MW | 244 |
Tested applications | ELISA, FC, IHC |
Dilution range | ELISA: 1:16000, IHC-P: 5 μg/ml |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.1. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti NOTCH3 antibody, anti Notch homolog 3 (Drosop Read more... |
Note | For research use only |
Entrez | 4854 |
Immunohistochemical staining of Human Hippocampus using NOTCH3 antibody
Immunohistochemical staining of Human Placenta using NOTCH3 antibody
Flow cytometric analysis of paraformaldehyde fixed HeLa cells using NOTCH3 antibody.
ELISA, IF | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |