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NOTCH3 antibody
Description
Images & Validation
−| Tested Applications | ICC, IF, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:100-500, IHC-P: 1:50-500, IF/ICC: 1:50-500 |
| Reactivity | Guinea pig, Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human NOTCH3. Please contact us for the exact immunogen sequence. The peptide is available as orb13850. |
| Target | NOTCH3 |
| Molecular Weight | 243 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
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IF analysis of rat lipocyte tissue using NOTCH3 antibody (2.5 ug/ml)

Immunohistochemical staining of rat lipocyte tissue using NOTCH3 antibody (2.5 ug/ml)

Western blot analysis of rat lipocyte (lane 1), rat pancreas (lane 2), mouse brain (lane 3), mouse colon (lane 4), human breast cancer (lane 5); hela cells (lane 6) using NOTCH3 antibody (1 ug/ml)

IF analysis of rat lipocyte tissue using NOTCH3 antibody (2.5 ug/ml)

Immunohistochemical staining of guinea pig pancreas tissue using NOTCH3 antibody (2.5 ug/ml)
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Protocol Information
NOTCH3 antibody (orb389328)
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