NOTCH3 Antibody

SKU: orb1247190

Description

NOTCH3 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.2. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Hippocampus shows select nuclear staining and weaker staining in different comparments of the neuronal cell bodies. Recommended concentration: 4-6µg/mL. Paraffin embedded Human Placenta. Recommended concentration: 5µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QLGPQPEVTPKRQ
• Target: NOTCH3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NOTCH3, Notch homolog 3 (Drosophila), HGNC:7883, CADASIL, CASIL , Notch (Drosophila) homolog 3, Notch homolog 3

Quick Database Links

Gene Symbol

NOTCH3

UniProt

Q9UM47

RefSeq (Protein):NP_000426.2