Mouse Nkx2-5 Antibody (Center)

• Catalog Number: orb1787994
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Immunogen: 98-133 aa
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, IHC: 1:25
• Conjugation: Unconjugated
• MW: 34163 Da
• Target: This mouse Nkx2-5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 98-133 amino acids from the Central region of mouse Nkx2-5.
• UniProt ID: P42582
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Homeobox protein Nkx-25, Cardiac-specific homeobox
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-Nkx2-5 Antibody (Center) at 1:2000 dilution. Lane 1: mouse spleen lysates. Lane 2: mouse stomach lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining Mouse Nkx2-5 in mouse spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.