Nigericin

SKU: orb1706910

Description

Nigericin is a polyether antibiotic with broad bioactivity, including antimicrobial, antimalarial, and anticancer properties. Its research applications involve studying ionophore-induced cell death mechanisms, such as pyroptosis and apoptosis via Caspase activation, in models like triple-negative breast cancer and primary effusion lymphoma, both in vitro and in vivo.

Research Area

Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Pharmacology & Drug Discovery, Protein Biochemistry, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 28380-24-7
• MW: 724.96
• Purity: 99.02% (May vary between batches)
• Formula: C₄₀H₆₈O₁₁
• SMILES: C[C@H]1[C@]2(O[C@@](C)(C1)[C@@]3(O[C@@](C)(CC3)[C@@]4(O[C@](C[C@@H]4C)([C@]5(O[C@](CO)(O)[C@H](C)C[C@@H]5C)[H])[H])[H])[H])O[C@H](C[C@@H]6O[C@@]([C@H](C(O)=O)C)([C@@H](C)CC6)[H])C[C@@H](OC)[C@H]2C
• Target: Pyroptosis,Antibacterial,Sodium Channel,NOD-like Receptor (NLR),Apoptosis,Caspase,Potassium Channel,Antibiotic,Wnt/beta-catenin
• Solubility: Ethanol:45.91 mg/mL (63.33 mM);DMSO:5 mg/mL (6.9 mM)

Bioactivity

• Target IC50:
  MDA-MB-231 cells:2.881 μM|4T1 cells:2.505 μM
• In Vivo:
  Methods: BALB/c mice transplanted with 4T1 cells were treated with Nigericin (2 mg/kg, subcutaneously, every two days for 4 weeks), and tumor volume was monitored every 3 to 4 days. At the end of the experiment, mice were euthanized by carbon dioxide, and tumors were harvested for further analysis. Results: Nigericin inhibited tumor growth.
• In Vitro:
  Methods: MDA-MB-231, MDA-MB-468, SK-BR-3, MCF-7 and 4T1 cells were treated with Nigericin (0.25, 0.5, 1, 2, 5, 10 and 20 μg/mL), cell viability was measured by MTS assay, and IC50 values ​​were calculated based on the cell viability curves. Results: Nigericin inhibited the cell viability of MDA-MB-231 and 4T1 cells in a dose-dependent manner, with IC50 values ​​of 2.881 μM and 2.505 μM, respectively.

Storage & Handling

• Storage: keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

K+/H+ ionophore, Nigericin

Compound Identifiers

PubChem CID

34230