NFE2L2/NRF2 Antibody

• Catalog Number: orb1294327
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NRF2
• Target: NFE2L2 / NRF2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:100-1:200 Immunohistochemistry (Paraffin) 1:100-1:200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cell Biology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Mina Kiani 1, Malek Soleimani Mehranjani 2, Mohammad Ali Shariatzadeh Empagliflozin reduces the adverse effects of diabetes mellitus on testicular tissue in type 2 diabetic Rats: A stereological and biochemical study Biochem Pharmacol ., (2024)

PubMed: 38508421

Applications

IHC

Reactivity

Rat

Anti-NRF2 antibody at 1/800 dilution. Lysates at 30 μg per lane. This blot was produced using a 5% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with orb1294327 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NRF2 with orb1294327 at 1/150.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NRF2 with orb1294327 at 1/150.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NRF2 with orb1294327 at 1/150.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294327 anti-NRF2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294327 anti-NRF2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse Kidney tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse lung labeling NRF2 with orb1294327 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse liver labeling NRF2 with orb1294327 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.