Nrf2 antibody

• Catalog Number: orb11165
• Category: Antibodies
• Description: Nrf2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-P, WB
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Nrf2. Please contact us for the exact immunogen sequence. The peptide is available as orb374740.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: WB:1:100-1000, IHC-P:1:100-600, IF/ICC: 1:100-600 (based on 0.5 mg/ml)
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 68 kDa
• Target: Nrf2
• Entrez: 4780
• UniProt ID: Q16236
• NCBI: 001138884, 21, 001145412, 11
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: anti erythroid derived 2 antibody, anti HEBP1 anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Khadrawy, Omar et al. Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function Int J Mol Sci, 20, (2019)

PubMed: 30986945

Applications

IF

Reactivity

Bovine

Liu, Guoyong et al. Astaxanthin attenuates adriamycin-induced focal segmental glomerulosclerosis Pharmacology, 95, 193-200 (2015)

PubMed: 25924598

Applications

WB

Reactivity

Mouse

Yu, Jian-bo et al. Role of Nrf2/ARE pathway in protective effect of electroacupuncture against endotoxic shock-induced acute lung injury in rabbits PLoS One, 9, e104924 (2014)

PubMed: 25115759

Applications

WB

Reactivity

Rabbit

Immunohistochemical staining of paraffin embedded rat liver tissue using anti-NRF2 (2.5 ug/ml)

Immunohistochemical staining of rat lung tissue using anti-NRF2 (2.5 ug/ml)

Western blot analysis of mouse skin (lane 1)

human lung cancer (lane 2)

293T cells (lane 3)

U251 cells (lane 4) using NRF2 antibody (1 ug/ml)

IHC-P staining of mouse kidney tissue using NRF2 antibody (2.5 ug/ml)

WB analysis of rat lung (lane 1), rat kidney (lane 2), mouse muscle (lane 3), rat liver (lane 4) using NRF2 antibody (1 ug/ml)

IHC-P image of rat lung tissue using NRF2 antibody (2.5 ug/ml)

WB analysis of mouse skin (lane 1)

human lung cancer (lane 2)

293T cells (lane 3)