Neuroligin 4 Antibody

SKU: orb150982

Description

Mouse Anti-Mouse Neuroligin-4 Monoclonal IgG1

Research Area

Disease Biomarkers, Neuroscience

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), IHC-P (1:100)
• Reactivity: Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-469 was sufficient for detection of Neuroligin 4 in 20 µg of mouse brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N98/7 (Formerly sold as S98-7)
• Immunogen: Fusion protein amino acids 782-945 (intracellular C-terminus) of mouse Neuroligin 4. ~45% identity with Neuroligin-1, -2 and -3.
• Target: Neuroligin 4
• Molecular Weight: 125-130kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.1% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ASPGX2, AUTSX2, HLNX, HNLX, KIAA1260, MGC22376, NLGN, NLGN4, NLGN4X, neuroligin-4 X-linked, neuroligin X, neuroligin 4, X-linked

Immunohistochemistry analysis using Mouse Anti-Neuroligin 4 Monoclonal Antibody, Clone N98/7. Tissue: Dentate Gyrus. Species: Mouse. Fixation: formaldehyde-fixed paraffin embedded. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain at 1:1000.

Western Blot analysis of Mouse Brain Membrane showing detection of ~125-130 kDa Neuroligin 4 protein using Mouse Anti-Neuroligin 4 Monoclonal Antibody, Clone N98/7. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Membrane. Load: 15 μg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~125-130 kDa.

Quick Database Links

Gene Symbol

Neuroligin 4

UniProt

B0F2B4

NCBI Gene ID

100113365

RefSeq:ABS19580