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Neuroligin 4 Antibody: APC
Catalog Number: orb150992
| Catalog Number | orb150992 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal to Neuroligin 4 (APC). This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. The encoded protein interacts with discs, large (Drosophila) homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. Two transcript variants encoding the same protein have been identified for this gene.. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | N98/7 (Formerly sold as S98-7) |
| Tested applications | ICC, IF, IHC |
| Reactivity | Mouse, Rat |
| Isotype | IgG1 |
| Immunogen | Fusion protein amino acids 782-945 (intracellular C-terminus) of mouse Neuroligin 4. ~45% identity with Neuroligin-1, -2 and -3. |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000), IHC (1:100) |
| Conjugation | APC |
| MW | 125-130kDa |
| Target | Neuroligin 4 |
| Entrez | 100113365 |
| UniProt ID | B0F2B4 |
| NCBI | ABS19580 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Alternative names | ASPGX2 antibody, AUTSX2 antibody, HLNX antibody, H Read more... |
| Note | For research use only |
| Application notes | 1 µg/ml of SMC-469 was sufficient for detection of Neuroligin 4 in 20 µg of mouse brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemistry analysis using Mouse Anti-Neuroligin 4 Monoclonal Antibody, Clone N98/7. Tissue: Dentate Gyrus. Species: Mouse. Fixation: formaldehyde-fixed paraffin embedded. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain at 1:1000.
Western Blot analysis of Mouse Brain Membrane showing detection of ~125-130 kDa Neuroligin 4 protein using Mouse Anti-Neuroligin 4 Monoclonal Antibody, Clone N98/7. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Membrane. Load: 15 μg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~125-130 kDa.
