Neuroligin 4 Antibody (PerCP)

SKU: orb150997

Description

Mouse monoclonal to Neuroligin 4 (PerCP). This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. The encoded protein interacts with discs, large (Drosophila) homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. Two transcript variants encoding the same protein have been identified for this gene..

Research Area

Disease Biomarkers, Neuroscience

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution range: WB (1:1000), IHC (1:100)
• Reactivity: Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-469 was sufficient for detection of Neuroligin 4 in 20 µg of mouse brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N98/7 (Formerly sold as S98-7)
• Immunogen: Fusion protein amino acids 782-945 (intracellular C-terminus) of mouse Neuroligin 4. ~45% identity with Neuroligin-1, -2 and -3.
• Target: Neuroligin 4
• Molecular Weight: 125-130kDa
• Purification: Protein G Purified
• Conjugation: PerCP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

ASPGX2, AUTSX2, HLNX, HNLX, KIAA1260, MGC22376, NLGN, NLGN4, NLGN4X, neuroligin-4 X-linked, neuroligin X, neuroligin 4, X-linked

Immunohistochemistry analysis using Mouse Anti-Neuroligin 4 Monoclonal Antibody, Clone N98/7. Tissue: Dentate Gyrus. Species: Mouse. Fixation: formaldehyde-fixed paraffin embedded. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain at 1:1000.

Western Blot analysis of Mouse Brain Membrane showing detection of ~125-130 kDa Neuroligin 4 protein using Mouse Anti-Neuroligin 4 Monoclonal Antibody, Clone N98/7. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Membrane. Load: 15 μg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~125-130 kDa.

Quick Database Links

Gene Symbol

Neuroligin 4

UniProt

B0F2B4

NCBI Gene ID

100113365

RefSeq:ABS19580