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KO/KD
Validated
Validated
Description
Research Area
Neuroscience
Images & Validation
−
| Tested Applications | ICC, IF, IHC-P, IP, KO/KD Validated, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | BT25X8M9 |
| Immunogen | A synthesized peptide derived from human NEFM |
| Target | NEFM |
| Molecular Weight | Calculated MW: 102 kDa; Observed MW: 160 kDa |
| Purification | Affinity Chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% rAlbumin, 0.02% sodium azide and 50% glycerol. |
| Buffer/Preservatives | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NEFM; NEF3; NFM; Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein
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Quick Database Links
Gene Symbol
NEFM
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ICC
Immunocytochemistry
IP
Immunoprecipitation
Available Sizes
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