Goat anti-NEFM / NF-M Antibody

• Catalog Number: orb20343
• Category: Antibodies
• Description: Goat polyclonal antibody to NEFM
• Target: NEFM / NF-M
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QASHITVERKDYLK
• RRID: AB_10749791
• MW: 102; 59.5
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NEFM antibody, anti neurofilament, medium pol
• Note: For research use only
• Entrez: 4741

orb20343 (0.03ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.