You have no items in your shopping cart.
Page Not Found
Cart summary
Item 1 of 3
Item 1 of 3
NEFM Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb1974710
| Catalog Number | orb1974710 |
|---|---|
| Category | Antibodies |
| Description | NEFM Recombinant Rabbit Monoclonal Antibody |
| Target | Nefm |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide derived from human NEFM |
| UniProt ID | P07197 |
| MW | 102 kDa |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, ICC/IF=1:100-200, IF=1:100-200 |
| Antibody Type | Recombinant Antibody |
| Clone Number | 7G3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Neurofilament medium polypeptide; 160 kDa neurofil Read more... |
| Note | For research use only |
Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (NEFM) Monoclonal Antibody, Unconjugated (orb1974710) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (NEFM) Monoclonal Antibody, Unconjugated (orb1974710) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human left frontal lobe), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (NEFM) Monoclonal Antibody, Unconjugated (orb1974710) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
