NeuN Antibody, KO Validated

• Catalog Number: orb1274564
• Category: Antibodies
• Description: NeuN Antibody, KO Validated
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NeuN (NP_001076044.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 50kDa
• Target: RBFOX3
• UniProt ID: A6NFN3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: FOX-3, FOX3, HRNBP3, NEUN, RNA binding protein fox
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using NeuN antibody at 1:1000 dilution.

Western blot analysis of extracts from normal (control) and NeuN knockout (KO) 293T cells, using NeuN antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat brain using NeuN antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using NeuN antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using NeuN antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-251MG cells using NeuN antibody at dilution of 1:100. Blue: DAPI for nuclear staining.