FOX3 (NeuN) Antibody

SKU: orb613671

Description

Our Anti-FOX3 (NeuN) primary antibody from Biorbyt is mouse monoclonal. It detects bovine, human, mouse, and rat FOX3 (NeuN) and is Protein G purified.

Research Area

Disease Biomarkers, Epigenetics & Chromatin, Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 1.430555556, IHC: 1:200-1:1000, ICC: 1:200-1:1000
• Reactivity: Bovine, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: 1B7
• Immunogen: Recombinant protein taken from the N-terminus of human FOX3 expressed in and purified from E. Coli.
• Target: FOX3 (NeuN)
• Molecular Weight: 46/48
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 50% glycerol and 5 mm NaN3
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FLJ56884 antibody, FLJ58356 antibody, Fox-1 homolog C antibody, fox1 homolog C antibody, Fox 3 antibody, FOX3NeuN antibody, hexaribonucleotide binding protein 3 antibody, HRNBP3 antibody, NeuN antibody, NEUN antibody, neuronal nuclei antibody, Rbfox3 antibody, RFOX3_HUMAN antibody, RNA binding protein fox-1 homolog 3 antibody, RNA binding protein fox 1 homolog (C. elegans) 3 antibody

Cell Line: human-derived iPSC cerebral organoids. MAP2 (pink), NeuN (yellow), DAPI (turquoise).

Cell Line: human-derived iPSC cerebral organoids. Neurofilament-H (green), NeuN (purple), DAPI (turquoise).

Immunofluorescence of a section of rat brain stem co-labeled with Anti-FOX3 (Cat. orb613671, green, 1:1000) and Anti-MAP2 (Cat. orb319566, red, 1:5000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.

Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L (Cat. orb319587, 1:2000, green) and labeling of FOX3/NeuN (Cat. orb613671, 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.

Immunofluorescence of a section of rat hippocampus showing specific labeling of UCHL1 (Cat. orb319704, 1:5000, green) in cell bodies and dendrites of neurons, and specific labeling of FOX3 (red). The blue is DAPI staining of nuclear DNA.

Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3 (Cat. orb613671, 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP (Cat. orb319550, 1:1000, green) and nuclear staining was done with DAPI (blue).

Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX3 protein.

Quick Database Links

Gene Symbol

FOX3 (NeuN)

UniProt

A6NFN3