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NeuN Rabbit Polyclonal Antibody

Catalog Number: orb500816

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb500816
CategoryAntibodies
DescriptionNeuN Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human NeuN (51-150/312aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW34 kDa
TargetRBFOX3
UniProt IDA6NFN3
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesRFOX3_HUMAN; RNA binding protein fox-1 homolog 3;
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NoteFor research use only
Expiration Date12 months from date of receipt.
NeuN Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

NeuN Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

NeuN Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

NeuN Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

NeuN Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

NeuN Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

NeuN Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

NeuN Rabbit Polyclonal Antibody

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-NeuN (orb500816) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46/50 kD, Observed band size: 46/50 kD.

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