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Description
Images & Validation
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Item 1 of 1
| Tested Applications | FC, ICC, IHC-P, IP, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try; WB: 1/5000. Predicted molecular weight: 177 kDa. ICC: 1/100 - 1/500. IHC(P): 1/500. Flow Cyt: 1/200. |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 10C2 |
| Immunogen | Human nestin protein corresponding to residues 1464-1614 |
| Target | Nestin |
| Purification | Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody containing PBS 0.1% sodium azide; |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ESTM 46 antibody;FLJ 21841 antibody;FLJ21841 antibody;Intermediate filament proteinantibody;Nbla00170 antibody;Nes antibody;NEST_HUMAN antibody;Nestin antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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