Mouse anti Nestin

• Catalog Number: orb98393
• Category: Antibodies
• Description: Mouse monoclonal antibody to Nestin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 10C2
• Tested applications: ICC, IHC-Fr, IHC-P, WB
• Reactivity: Human, Monkey, Primate
• Isotype: IgG1
• Antibody Type: Primary Antibody
• Form/Appearance: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Biorbyt accepts no liability for any inaccuracies or omissions in this information.
• UniProt ID: P48681
• Source: Monoclonal antibody 10C2 was derived by fusion of P3x63Ag8.653 myeloma cells with spleen cells from a BALB/c mouse immunized with a 150-amino-acid fragment from the cloned human nestin, conjugated to glutathione S-transferase.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunostaining of intracellular nestin filaments in cultured SH-SY-5Y neuronal cells using orb98393 (1:100 dilution).

orb98393 Immunostaining of intracellular nestin filaments in U251 cells

orb98393 Immunoblotting of U251-cell lysate showing reactivity with nestin