Nestin Mouse Monoclonal Antibody

SKU: orb1816769

Description

Nestin Mouse Monoclonal Antibody

Research Area

Angiogenesis, Angiogenic Factors, Cancer Research, Cardiovascular Research, Cell Biology, Cell Markers, Cell Signaling, Intercellular Signaling, Intermediate Filaments, Neurogenesis, Neuronal restricted progenitors (NRPs), Neuroscience, Stem Cell Research

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: IHC-P=1:100-500, WB=1:500-1000, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, κ
• Clone No.: E2G4
• Immunogen: KLH conjugated synthetic peptide derived from human Nestin
• Target: NES
• Molecular Weight: 178 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, Glycerol, rAlbumin.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_HUMAN; Nestin.

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Nestin) Monoclonal Antibody, Unconjugated (orb1816769) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Nestin) Monoclonal Antibody, Unconjugated (orb1816769) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Nestin) Monoclonal Antibody, Unconjugated (orb1816769) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Quick Database Links

Gene Symbol

NES

UniProt

P48681