NELFE Antibody

SKU: orb1257907

Description

NELFE Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NELFE (NP_002895.3).
• Target: NELFE
• Molecular Weight: Observed: 43kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

D6S45, NELF-E, RD, RDBP, RDP, negative elongation factor E, RD RNA-binding protein, RNA-binding protein RD, major histocompatibility complex gene RD, negative elongation factor polypeptide E, nuclear protein

Western blot analysis of extracts of various cell lines, using NELFE antibody (orb1257907) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human lung cancer using NELFE antibody (orb1257907) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse Intestine using NELFE antibody (orb1257907) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using NELFE antibody (orb1257907) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A-549 cells using NELFE antibody (orb1257907).

Quick Database Links

Gene Symbol

NELFE

UniProt

P18615