NELF Antibody

• Catalog Number: orb1239656
• Category: Antibodies
• Description: NELF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Rabbit polyclonal NELF antibody was raised against a 17 amino acid peptide near the center of human NELF.The immunogen is located within amino acids 230 - 280 of NELF.
• Concentration: 1 mg/mL
• Dilution range: NELF antibody can be used for detection of NELF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 58 kDa
• Target: NELF
• UniProt ID: Q6X4W1
• NCBI: NP_056352
• Storage: NELF antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: NELF Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: NELF Antibody: HH9, NELF, NMDA receptor synaptonuc
• Note: For research use only
• Application notes: NELF antibody can be used for detection of NELF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NELF in Hela cell lysate with NELF antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of NELF in rat brain tissue with NELF antibody at 2.5 µg/ml.

Immunofluorescence of NELF in rat brain tissue with NELF antibody at 20 µg/ml.

Immunohistochemistry of NELF in mouse brain tissue with NELF Antibody at 5 µg/mL.

Immunofluorescence of NELF in mouse brain tissue with NELF Antibody at 20 µg/mL. Green: NELF antibody (orb1239656) Red: Phylloidin staining Blue: DAPI staining.