NELF Antibody

SKU: orb1239656

Description

NELF Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  NELF antibody can be used for detection of NELF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Rabbit polyclonal NELF antibody was raised against a 17 amino acid peptide near the center of human NELF.The immunogen is located within amino acids 230 - 280 of NELF.
• Target: NELF
• Molecular Weight: Predicted: 58 kDa
• Purification: NELF Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: NELF Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

NELF Antibody: HH9, NELF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor

Western blot analysis of NELF in Hela cell lysate with NELF antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of NELF in rat brain tissue with NELF antibody at 2.5 µg/ml.

Immunofluorescence of NELF in rat brain tissue with NELF antibody at 20 µg/ml.

Immunohistochemistry of NELF in mouse brain tissue with NELF Antibody at 5 µg/mL.

Immunofluorescence of NELF in mouse brain tissue with NELF Antibody at 20 µg/mL. Green: NELF antibody (orb1239656) Red: Phylloidin staining Blue: DAPI staining.

Quick Database Links

Gene Symbol

NELF

UniProt

Q6X4W1

RefSeq (Protein):NP_056352