NEFM Antibody

SKU: orb1259019

Description

NEFM Antibody

Research Area

Cell Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NEFM (NP_005373.2).
• Target: NEFM
• Molecular Weight: Observed: 140kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NEFM, neurofilament-3 (150 kD medium), neurofilament, medium poly peptide 150 kDa, neurofilament triplet M protein, NFM, NF-M, NEF3, medium poly peptide, NEFM

Western blot analysis of extracts of various cell lines, using NEFM antibody (orb1259019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of mouse brain using NEFM antibody (orb1259019) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat brain using NEFM antibody (orb1259019) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

NEFM

UniProt

P07197