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NEDD8 Antibody
Description
Images & Validation
−| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 5B8C5 |
| Immunogen | Purified recombinant fragment of human NEDD8 expressed in E. Coli. |
| Target | NEDD8 |
| Molecular Weight | 9kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
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Immunohistochemical analysis of formalin fixed and paraffin-embedded prostate cancer tissues using NEDD8 antibody

Western blot analysis of C6 (Lane1) and Hela (Lane2) cell lysate using NEDD8 antibody

Flow cytometric analysis of Hela cells using NEDD8 antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of NEDD8 antibody

Confocal immunofluorescence analysis of Hela cells using NEDD8 antibody

Western blot analysis of human NEDD8 (AA: 1-81) recombinant protein using NEDD8 antibody
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Protocol Information
NEDD8 Antibody (orb97420)
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