Anti-NUB1 Antibody

• Catalog Number: orb1786115
• Category: Antibodies
• Description: Anti-NUB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: E.coli-derived human NUB1 recombinant protein (Position: K190-D543).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry, 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 75 kDa
• UniProt ID: Q9Y5A7
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-NUB1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-NUB1 antibody.1:HeLa cell;2:Colo320 cell;3:human HepG2 cell;4:human K562 cell;5:human Raji cell;6:monkey COS-7 cell;8:human MCF-7 cell;9:human A549 cell;10:rat liver tissue;11:rat PC-12 cell;12:mouse NIH/3T3 cell.

IF analysis of NUB1 using anti-NUB1 antibody and anti-Beta Tubulin antibody. NUB1 was detected in immunocytochemical section of HELA cell.

IHC analysis of NUB1 using anti-NUB1 antibody. NUB1 was detected in a paraffin-embedded section of human adrenal adenomas tissue.

IHC analysis of NUB1 using anti-NUB1 antibody. NUB1 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue.

IHC analysis of NUB1 using anti-NUB1 antibody. NUB1 was detected in a paraffin-embedded section of human ovarian cancer tissue.