UBE1C Antibody (C-term)

• Catalog Number: orb1938834
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB2803
• Tested applications: IHC-P, WB
• Predicted Reactivity: Rat, Zebrafish
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 51852 Da
• Target: This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-445 amino acids from the C-terminal region of human UBE1C.
• UniProt ID: Q8TBC4
• NCBI: NP_003959.3, NP_937838.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: NEDD8-activating enzyme E1 catalytic subunit, 632-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-UBE1 C-term Pab is used in Western blot to detect UBE1 in mouse brain tissue lysate.

UBE1C Antibody (S430) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the UBE1C antibody detected the UBE1C protein (arrow).

Western blot analysis of UBE1C (arrow) using rabbit polyclonal UBE1C Antibody (S430). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE1C gene.