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MYD88 Antibody

Catalog Number: orb1239647

DispatchUsually dispatched within 5-10 working days
$ 670.00
Catalog Numberorb1239647
CategoryAntibodies
DescriptionMYD88 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC-P, IP, WB
Predicted ReactivityBovine, Gallus, Porcine, Sheep
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenAnti-MYD88 antibody (orb1239647) was raised against a peptide corresponding to 16 amino acids near the center of human MYD88 isoform 1. The immunogen is located within amino acids 220 - 270 of MYD88.
Antibody TypePrimary Antibody
Concentration1 mg/mL
Form/AppearanceLiquid
ConjugationUnconjugated
MWPredicted: 35kD Observed: 35kD
TargetMYD88
UniProt IDU70451
NCBIAAB49967.1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesMYD88 Antibody is supplied in PBS containing 0.02% sodium azide.
Alternative namesMYD88 Antibody: Myeloid differentiation primary re
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NoteFor research use only
Expiration Date12 months from date of receipt.
MYD88 Antibody

Western Blot Validation of MyD88 in HeLa (A) and Jurket (B) Cells. Loading: 15 µg of lysates per lane. Antibodies: orb1239647 (1 µg/mL) 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

MYD88 Antibody

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: MyD88 orb1239647 (2 µg/mL), MyD88 orb1239635 (2 µg/mL), beta-actin (1 µg/mL), and GAPDH (0.02 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

MYD88 Antibody

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues. Loading: 15 µg of lysates per lane. Antibodies: MyD88 orb1239647 (2 µg/mL), MyD88 orb1239635 (2 µg/mL), beta-actin (1 µg/mL), and GAPDH (0.02 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

MYD88 Antibody

Animal Species Reactivity. Loading: Lysates/proteins at 15 µg per lane. Antibodies: orb1239647 (2 µg/mL) or orb1239635 (2 µg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

MYD88 Antibody

Validation with MyD88 siRNA Knockdown in HeLa Cells. HeLa cells were transfected with control siRNAs (lane 1) or MyD88 siRNAs (lane 2) Loading: 10 µg of HeLa whole cell lysates per lane. Antibodies: orb1239647 (2 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

MYD88 Antibody

Immunofluorescence Validation of MyD88 n Human Testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling MyD88 with orb1239647 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing nucleus staining on human testis cells.

MYD88 Antibody

Immunohistochemistry Validation of MyD88 in Human Heart. Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-MyD88 antibody (orb1239647) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

MYD88 Antibody

Immunofluorescence Validation of MyD88 in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling MyD88 with orb1239647 at 10 µg/mL, followed by Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

MYD88 Antibody

KO Validation in Mouse Macrophages (Miller et al., 2006). Bone marrow-derived macrophages from wild type (WT) mice and MyD88 knockout mice were assessed for MyD88 protein expression by anti-MyD88 antibodies. MyD88 expression was detected in WT mice, but not in MyD88 knockout mice.

MYD88 Antibody

KO Validation in MyD88-deficient MEF cell line (Burns et al., 2003). MyD88−/− deficient MEF cell line was reconstituted by retroviral infection with an empty vector, MyD88, or MyD88s expression vectors. The levels of MyD88 (isoform 1) or MyD88s (isoform 3) were confirmed with anti-MyD88 antibodies and MyD88 expression was not detected in the MyD88-deficient cells.

MYD88 Antibody

KO Validation in Mouse IECs (Vlantis et al., 2014). Western blot with anti-MyD88 antibodies on intestinal epithelial cells (IECs) showing efficient deletion of MyD88 and concomitant expression of GFP in MyD88IEC-KO mice, but not in MyD88 knockout mice.

MYD88 Antibody

KD Validation in Chondrocytes (Ahmad et al., 2009). Chondrocytes were transfected with either MyD88 siRNA or control siRNA and analyzed for MyD88 expression by immunoblotting with anti-Myd88 antibodies that confirmed inhibition of the target proteins.

MYD88 Antibody

Immunoprecipitation Validation in HEK293 cells (Kawai et al., 2004). HEK293 cells were transiently transfected with FLAG-IRF7. Cell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-FLAG.

  • MYD88 Antibody [orb1239635]

    ELISA,  IF,  IP,  WB

    Bovine, Gallus, Porcine, Sheep

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    0.1 mg
  • Anti-MyD88 Antibody [orb215902]

    ELISA,  FC,  ICC,  IF,  IHC,  IHC-Fr,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Goat anti-MYD88 Antibody [orb18963]

    ELISA,  FC,  IF,  IHC,  WB

    Canine, Human, Mouse, Rat

    Goat

    Polyclonal

    Unconjugated

    100 μg
  • MyD88 Rabbit Polyclonal Antibody [orb11091]

    FC,  IF,  IHC-Fr,  IHC-P,  WB

    Human, Rat

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl, 200 μl
  • MYD88 antibody [orb420074]

    IHC-P,  WB

    Guinea pig, Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg