MYD88 Antibody

• Catalog Number: orb1239635
• Category: Antibodies
• Description: MYD88 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IP, WB
• Predicted Reactivity: Bovine, Gallus, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-MYD88 antibody (orb1239635) was raised against a peptide corresponding to 17 amino acids near carboxy terminus of human MYD88 isoform 1. The immunogen is located within the last 50 amino acids of MYD88.
• Concentration: 1 mg/mL
• Dilution range: WB: 2 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence and Immunoprecipitation in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 35kD Observed: 35kD
• Target: MYD88
• UniProt ID: U70451
• NCBI: AAB49967.1
• Storage: MYD88 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: MYD88 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: MYD88 Antibody: MYD88D
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

KO Validation in HeLa Cells. Loading: 10 µg of HeLa WT cell lysate or MyD88 KO cell lysate. Antibodies: MyD88 orb1239635 (2 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation of MyD88 in human cell lines. Loading: 15 µg of lysates per lane. Antibodies: orb1239635 (2 µg/mL) 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Predicted band size: 35 kDa.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: MyD88 orb1239647 (2 µg/mL), MyD88 orb1239635 (2 µg/mL), beta-actin (1 µg/mL), and GAPDH (0.02 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues. Loading: 15 µg of lysates per lane. Antibodies: MyD88 orb1239647 (2 µg/mL), MyD88 orb1239635 (2 µg/mL), beta-actin (1 µg/mL), and GAPDH (0.02 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Animal Species Reactivity. Loading: Lysates/proteins at 15 µg per lane. Antibodies: orb1239647 (2 µg/mL) or orb1239635 (2 µg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of MyD88 in Jurkat Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling MyD88 with orb1239635 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Immunofluorescence Validation of MyD88 in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling MyD88 with orb1239635 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

KO Validation of MyD88 in Mouse T cells (Rahman et al., 2011). Splenocytes were isolated from a Myd88 Delta T mouse in which MyD88 was specifically disrupted in T cells. T and B cells were FACS purified, and MyD88 expression was examined by Western blot with anti-MyD88 antibodies (orb1239635). MyD88 expression was detected in Splenocytes and B cells, but not in T cells.

KO Validation in CD45 - Thy1 + cells (Kim et al., 2017). The expression of Myd88 protein was analyzed in CD45 - Thy1 + intestinal mesenchymal cells and CD3 + intestinalT cells, by immunoblotting with anti-Myd88 antibodies (orb1239635). MyD88 expression was not detected in knockout cells.

KD Validation in Raw 264.7 cells (Altimeier et al., 2007). The Transfection of RAW 264.7 cells with MyD88-specific siRNAresulted in attenuation of MyD88 protein by Western blot analysis with anti-Myd88 antibodies (orb1239635).

Immunoprecipitation Validation in HEK293 cells (Kawai et al., 2004). HEK293 cells were transiently transfected with FLAG-IRF7. Cell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-FLAG.