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MYD88 Antibody

Catalog Number: orb1239635

DispatchUsually dispatched within 5-10 working days
$ 670.00
Catalog Numberorb1239635
CategoryAntibodies
DescriptionMYD88 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IF, IP, WB
Predicted ReactivityBovine, Gallus, Porcine, Sheep
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenAnti-MYD88 antibody (orb1239635) was raised against a peptide corresponding to 17 amino acids near carboxy terminus of human MYD88 isoform 1. The immunogen is located within the last 50 amino acids of MYD88.
Antibody TypePrimary Antibody
Concentration1 mg/mL
Form/AppearanceLiquid
ConjugationUnconjugated
MWPredicted: 35kD Observed: 35kD
TargetMYD88
UniProt IDU70451
NCBIAAB49967.1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesMYD88 Antibody is supplied in PBS containing 0.02% sodium azide.
Alternative namesMYD88 Antibody: MYD88D
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NoteFor research use only
Expiration Date12 months from date of receipt.
MYD88 Antibody

Animal Species Reactivity. Loading: Lysates/proteins at 15 µg per lane. Antibodies: orb1239647 (2 µg/mL) or orb1239635 (2 µg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

MYD88 Antibody

Immunofluorescence Validation of MyD88 In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling MyD88 with orb1239635 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

MYD88 Antibody

Immunofluorescence Validation of MyD88 in Jurkat Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling MyD88 with orb1239635 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

MYD88 Antibody

Immunohistochemistry Validation of MyD88 in Human Colon. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MYD88 antibody (orb1239635) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

MYD88 Antibody

Immunohistochemistry Validation of MyD88 in Mouse Heart. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-MYD88 antibody (orb1239635) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

MYD88 Antibody

Immunohistochemistry Validation of MyD88 in Rat Liver. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-MYD88 antibody (orb1239635) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

MYD88 Antibody

Immunoprecipitation Validation in HEK293 cells. HEK293 cells were transiently transfected with FLAG-IRF7. Cell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-FLAG.

MYD88 Antibody

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: MyD88 orb1239647 (2 µg/mL), MyD88 orb1239635 (2 µg/mL), beta-actin (1 µg/mL), and GAPDH (0.02 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

MYD88 Antibody

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues. Loading: 15 µg of lysates per lane. Antibodies: MyD88 orb1239647 (2 µg/mL), MyD88 orb1239635 (2 µg/mL), beta-actin (1 µg/mL), and GAPDH (0.02 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

MYD88 Antibody

KD Validation in Raw 264.7 cells. The Transfection of RAW 264.7 cells with MyD88-specific siRNA resulted in attenuation of MyD88 protein by Western blot analysis with anti-Myd88 antibodies (orb1239635).

MYD88 Antibody

KO Validation in HeLa Cells. Loading: 10 µg of HeLa WT cell lysate or MyD88 KO cell lysate. Antibodies: MyD88 orb1239635 (2 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

MYD88 Antibody

KO Validation of MyD88 in Mouse T cells. Splenocytes were isolated from a Myd88ΔT mouse in which MyD88 was specifically disrupted in T cells. T and B cells were FACS purified, and MyD88 expression was examined by Western blot with anti-MyD88 antibodies (orb1239635). MyD88 expression was detected in Splenocytes and B cells, but not in T cells.

MYD88 Antibody

Western Blot Validation of MyD88 in human cell lines. Loading: 15 µg of lysates per lane. Antibodies: orb1239635 (2 µg/mL) 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Predicted band size: 35 kDa.

  • MYD88 Antibody [orb1239647]

    ELISA,  IF,  IHC-P,  IP,  WB

    Bovine, Gallus, Porcine, Sheep

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    0.1 mg
  • Anti-MyD88 Antibody [orb215902]

    ELISA,  FC,  ICC,  IF,  IHC,  IHC-Fr,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Goat anti-MYD88 Antibody [orb18963]

    ELISA,  FC,  IF,  IHC,  WB

    Canine, Human, Mouse, Rat

    Goat

    Polyclonal

    Unconjugated

    100 μg
  • MyD88 Rabbit Polyclonal Antibody [orb11091]

    FC,  IF,  IHC-Fr,  IHC-P,  WB

    Human, Rat

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl, 200 μl
  • MYD88 antibody [orb420074]

    IHC-P,  WB

    Guinea pig, Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg