Cart summary

You have no items in your shopping cart.

Anti-MyD88 Antibody

Catalog Number: orb215902

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb215902
CategoryAntibodies
DescriptionAnti-MyD88 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, FC, ICC, IF, IHC, IHC-Fr, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenE.coli-derived human MyD88 recombinant protein (Position: A44-F264). Human MyD88 shares 84% and 83% amino acid (aa) sequences identity with mouse and rat MyD88, respectively.
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW33 kDa
UniProt IDP35354
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesMyeloid differentiation primary response protein M
Read more...
NoteFor research use only
Application notesWestern blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Immunofluorescence, 2μg/ml Flow Cytometry (Fixed), 1-3μg/1x106 cells ELISA (Cap), 1-5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-MyD88 Antibody

Flow Cytometry analysis of A549 cells using anti-MYD88 antibody. Overlay histogram showing A549 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYD88 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-MyD88 Antibody

IF analysis of MYD88 using anti-MYD88 antibody. MYD88 was detected in paraffin-embedded section of human colon cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-MyD88 Antibody

IF analysis of MYD88 using anti-MYD88 antibody. MYD88 was detected in paraffin-embedded section of human colon cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-MyD88 Antibody

IF analysis of MYD88 using anti-MYD88 antibody. MYD88 was detected in paraffin-embedded section of human mammary cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-MyD88 Antibody

IF analysis of MYD88 using anti-MYD88 antibody. MYD88 was detected in paraffin-embedded section of human mammary cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-MyD88 Antibody

IF analysis of MYD88 using anti-MYD88 antibody. MYD88 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-MyD88 Antibody

IF analysis of MYD88 using anti-MYD88 antibody. MYD88 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-MyD88 Antibody

IHC analysis of MYD88 using anti-MYD88 antibody. MYD88 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-MYD88 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-MyD88 Antibody

IHC analysis of MYD88 using anti-MYD88 antibody. MYD88 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-MYD88 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-MyD88 Antibody

IHC analysis of MYD88 using anti-MYD88 antibody. MYD88 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-MYD88 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-MyD88 Antibody

Western blot analysis of MYD88 using anti-MYD88 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human MYD88 Protein 0.5 ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYD88 at approximately 49 KD. The expected band size for MYD88 is at 49 KD.

Anti-MyD88 Antibody

Western blot analysis of MYD88 using anti-MYD88 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Rat Cardiac Muscle Tissue Lysate. Lane 2: HELA Whole Cell Lysate. Lane 3: MCF Whole Cell Lysate. Lane 4: HEPG2 Whole Cell Lysate. Lane 5: JURKAT Whole Cell Lysate. Lane 6: RAJI Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYD88 at approximately 33 KD. The expected band size for MYD88 is at 33 KD.

  • Goat anti-MYD88 Antibody [orb18963]

    ELISA,  FC,  IF,  IHC,  WB

    Canine, Human, Mouse, Rat

    Goat

    Polyclonal

    Unconjugated

    100 μg
  • Anti-MYD88 Antibody [orb214286]

    IF,  IH,  WB

    Human, Mouse, Porcine, Primate, Rat, Sheep

    Rabbit

    Polyclonal

    Unconjugated

    30 μl, 100 μl, 200 μl
  • Goat anti-MYD88, Biotinylated Antibody [orb334076]

    ELISA,  IHC,  WB

    Human

    Goat

    Polyclonal

    Unconjugated

    100 μg
  • Anti-MYD88 Antibody [orb1145807]

    ELISA,  FC,  IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-MyD88 Monoclonal Antibody [orb546988]

    FC,  ICC,  IF,  IHC,  WB

    Human

    Rabbit

    Monoclonal

    Unconjugated

    30 μl, 100 μl