IL17a Antibody

SKU: orb345240

Description

IL-17A antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, Multiplex Assay, WB
• Dilution Range: ELISA: 1:10,000, FC: 1:500, WB: 1:1000
• Reactivity: Mouse
• Application Notes:
  IL-17A monoclonal antibody has been tested for use in western blotting, flow cytometry and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23 kDa in size corresponding to the mature mouse Il17A protein, a non-glycosylated polypeptide chain consisting of 207 amino acids, by western blotting in appropriate cell lysate or extract.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 20B4.G10.F5
• Immunogen: This Protein A purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.
• Target: IL17a
• Purity: Anti-IL-17A is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse Il-17a protein. Cross-reactivity with Il-17a from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.94 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rat anti-IL-17A antibody, rat anti-interleukin17A antibody, Interleukin-17A cytokine, IL-17A, IL-17, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8

Quick Database Links

Gene Symbol

IL17a

UniProt

Q62386

RefSeq (Protein):NP_034682.1