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Nestin Rabbit Polyclonal Antibody
Description
Images & Validation
−| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:50-500, IHC-P: 1:100-500 (based on 0.5 mg/ml) |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Nestin. Please contact us for the exact immunogen sequence. The peptide is available as orb374738. |
| Target | Nestin. Recognized endogenous levels of total Nestin |
| Molecular Weight | 177 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Immunohistochemical staining of mouse bone marrow tissue using anti-Nestin (2.5 ug/ml)

Western blot analysis of U251 cells using Nestin antibody (3 ug/ml)

Immunohistochemical staining of paraffin embedded rat kidney tissue using Nestin antibody (5 ug/ml)

IHC-P image of rat kidney tissue using Nestin antibody (2.5 ug/ml)

IHC-P image of rat kidney tissue using Nestin antibody (2.5 ug/ml)

IHC-P staining of rat kidney tissue using Nestin antibody (2.5 ug/ml)

IHC-P image of rat kidney tissue using Nestin antibody (5 ug/ml)

IHC-P image of rat brain tissue using anti-Nestin (5 ug/ml)

IHC-P staining of rat pancreas tissue using anti-Nestin (2.5 ug/ml)

Immunohistochemical staining of rat brain tissue using Nestin antibody (5 ug/ml)

IHC-P staining of rat bone marrow tissue using Nestin antibody (2.5 ug/ml)
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Nestin Rabbit Polyclonal Antibody (orb11109)
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