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MonoMethyl-Histone H3-K9 pAb

Catalog Number: orb1258190

DispatchUsually dispatched within 5-10 working days
$ 960.00
Catalog Numberorb1258190
CategoryAntibodies
DescriptionMonoMethyl-Histone H3-K9 pAb
TargetH3K9me1
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Other, Rat
Form/AppearanceLiquid
Concentrationbatch dependent
Buffer/PreservativesLiquid
ImmunogenA synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
UniProt IDQ16695
MWObserved: 18kDa
Tested applicationsChIP, IF, IHC, IP, WB
Application notesWB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesH3F3A, H3t, H3.4, H3/g, H3FT
NoteFor research use only
MonoMethyl-Histone H3-K9 pAb

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K9 antibody (orb1258190). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

MonoMethyl-Histone H3-K9 pAb

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K9 antibody (orb1258190).

MonoMethyl-Histone H3-K9 pAb

Immunohistochemistry of paraffin-embedded rat testis using MonoMethyl-Histone H3-K9 antibody (orb1258190) at dilution of 1:200 (40x lens).

MonoMethyl-Histone H3-K9 pAb

Immunohistochemistry of paraffin-embedded human embryo brain using MonoMethyl-Histone H3-K9 antibody (orb1258190) at dilution of 1:200 (40x lens).

MonoMethyl-Histone H3-K9 pAb

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K9 antibody (orb1258190). Blue: DAPI for nuclear staining.

MonoMethyl-Histone H3-K9 pAb

Immunofluorescence analysis of C6 cells using MonoMethyl-Histone H3-K9 antibody (orb1258190) at dilution of 1:100. Blue: DAPI for nuclear staining.

MonoMethyl-Histone H3-K9 pAb

Immunofluorescence analysis of HeLa cells using MonoMethyl-Histone H3-K9 antibody (orb1258190) at dilution of 1:100. Blue: DAPI for nuclear staining.

MonoMethyl-Histone H3-K9 pAb

Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K9 antibody (orb1258190) at dilution of 1:100. Blue: DAPI for nuclear staining.

MonoMethyl-Histone H3-K9 pAb

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using MonoMethyl-Histone H3-K9 antibody (orb1258190) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.