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MonoMethyl-Histone H3-K9 pAb

Catalog Number: orb1258190

DispatchUsually dispatched within 5-10 working days
$ 960.00
Catalog Numberorb1258190
CategoryAntibodies
DescriptionMonoMethyl-Histone H3-K9 pAb
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsChIP, IF, IHC, IP, WB
ReactivityHuman, Mouse, Other, Rat
IsotypeIgG
ImmunogenA synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
Antibody TypePrimary Antibody
Concentrationbatch dependent
Form/AppearanceLiquid
ConjugationUnconjugated
MWObserved: 18kDa
TargetH3K9me1
UniProt IDQ16695
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid
Alternative namesH3F3A, H3t, H3.4, H3/g, H3FT
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NoteFor research use only
Application notesWB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
Expiration Date12 months from date of receipt.
MonoMethyl-Histone H3-K9 pAb

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K9 antibody (orb1258190). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

MonoMethyl-Histone H3-K9 pAb

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K9 antibody (orb1258190).

MonoMethyl-Histone H3-K9 pAb

Immunohistochemistry of paraffin-embedded rat testis using MonoMethyl-Histone H3-K9 antibody (orb1258190) at dilution of 1:200 (40x lens).

MonoMethyl-Histone H3-K9 pAb

Immunohistochemistry of paraffin-embedded human embryo brain using MonoMethyl-Histone H3-K9 antibody (orb1258190) at dilution of 1:200 (40x lens).

MonoMethyl-Histone H3-K9 pAb

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K9 antibody (orb1258190). Blue: DAPI for nuclear staining.

MonoMethyl-Histone H3-K9 pAb

Immunofluorescence analysis of C6 cells using MonoMethyl-Histone H3-K9 antibody (orb1258190) at dilution of 1:100. Blue: DAPI for nuclear staining.

MonoMethyl-Histone H3-K9 pAb

Immunofluorescence analysis of HeLa cells using MonoMethyl-Histone H3-K9 antibody (orb1258190) at dilution of 1:100. Blue: DAPI for nuclear staining.

MonoMethyl-Histone H3-K9 pAb

Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K9 antibody (orb1258190) at dilution of 1:100. Blue: DAPI for nuclear staining.

MonoMethyl-Histone H3-K9 pAb

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using MonoMethyl-Histone H3-K9 antibody (orb1258190) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.