Mono-Methyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody

• Catalog Number: orb1172463
• Category: Antibodies
• Description: Mono-Methyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody
• Target: H3C1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Predicted Reactivity: Monkey, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Antigen affinity purification
• MW: 17 kDa
• Tested applications: ICC, IP, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100-500, IP=1:25-100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics and Nuclear Signaling
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Pharbitis, N2a, Mouse spleen, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 17 kDa, Observed MW: 17 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: HeLa, 3T3, COS, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 17 kDa, Observed MW: 17 kDa.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:200, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1172463.