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Histone H3 K9me1 antibody

Histone H3 K9me1 antibody

Catalog Number: orb420396

DispatchUsually dispatched within 5-10 working days
$ 815.00
Catalog Numberorb420396
CategoryAntibodies
DescriptionHistone H3 K9me1 antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsChIP, DOT, IF, IHC, Multiplex Assay, WB
ReactivityC. elegans, Human, Mouse
IsotypeIgG
ImmunogenHistone H3 [Monomethyl Lys9] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 9 of human Histone H3.2.
Concentration0.7 mg/mL
Dilution rangeChIP: 2-5µg/million cells, IHC: 1:500, IF: 1:500, WB: 1:500
Form/AppearanceLiquid (sterile filtered)
PurityAnti-Histone H3 [Monomethyl Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
ConjugationUnconjugated
UniProt IDQ71DI3
NCBINP_001005464
StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesrabbit anti-Histone H3 monomethyl Lys9 antibody, H
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NoteFor research use only
Application notesAnti-Histone H3 [Monomethyl Lys9] antibody is tested for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunocytochemistry/Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.
Expiration Date12 months from date of receipt.
  • Histone H3 K9me1/pT6 antibody [orb420391]

    ChIP,  DOT,  IF,  IHC,  WB

    C. elegans, Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    50 μg
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