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    Mesothelin antibody

    Catalog Number: orb344442

    DispatchUsually dispatched within 5-10 working days
    $ 799.00
    Catalog Numberorb344442
    CategoryAntibodies
    DescriptionMesothelin antibody
    Species/HostMouse
    ClonalityMonoclonal
    Clone NumberMB-G10
    Tested applicationsELISA, FC, IHC, WB
    ReactivityHuman
    IsotypeIgG2a
    ImmunogenThis antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
    Concentration1.0 mg/mL
    Dilution rangeELISA: 1:10,000 - 1:50,000, FC: 1:200, IHC: 1:100, WB: 1:1,000
    Form/AppearanceLiquid (sterile filtered)
    PurityThis antibody is directed against human mesothelin protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. Cross reactivity with homologues from other sources has not been tested.
    ConjugationUnconjugated
    UniProt IDQ13421
    NCBI53988378
    StorageStore antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesmouse anti-Mesothelin Antibody, Mesothelian, MN, M
    Read more...
    NoteFor research use only
    Application notesThis antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For Anti-mesothelin immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.
    Expiration Date12 months from date of receipt.
    Mesothelin antibody

    Western blot analysis of 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5). Lane 6 contains 50 ng using Mesothelin antibody

    Mesothelin antibody

    Immunohistochemical staining of PEFF human tissue using Mesothelin antibody

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