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Catalog Number | orb344442 |
---|---|
Category | Antibodies |
Description | Mesothelin antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | MB-G10 |
Tested applications | ELISA, FC, IHC, WB |
Reactivity | Human |
Isotype | IgG2a |
Immunogen | This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:10,000 - 1:50,000, FC: 1:200, IHC: 1:100, WB: 1:1,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This antibody is directed against human mesothelin protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. Cross reactivity with homologues from other sources has not been tested. |
Conjugation | Unconjugated |
UniProt ID | Q13421 |
NCBI | 53988378 |
Storage | Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | mouse anti-Mesothelin Antibody, Mesothelian, MN, M Read more... |
Note | For research use only |
Application notes | This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For Anti-mesothelin immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5). Lane 6 contains 50 ng using Mesothelin antibody
Immunohistochemical staining of PEFF human tissue using Mesothelin antibody
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IHC, IHC-Fr, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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